CAS: 1239-45-8|Ethidium Bromide yog ib qho khoom noj khoom haus

Lub npe: Ethidium bromide

Synonyms: EtBr; Dromilac; 3, 8-Diamino-5-ethyl-6-phenylphenanthridinium bromide

CAS: 1239-45-8

Purity: 98 feem pua

Molecular Formula: C₂₁H₂₀N₃•Br

Molecular Luj: 394.3

Melting Point: 260-262 degree C (lit.)(dec.)

Boiling Point: N / A

Qhov ceev: 1.3739g / cm3³(nrog kwv yees)

Cov tsos mob: Tsaus Liab heev rau Tsaus Ntshav Ntshav Khoom

Stability: ruaj khov. Incompatible nrog muaj zog oxidizing neeg sawv cev.

Solubility: dej (10 mg / mL), DMSO (me ntsis), Methanol (me ntsis), Dej (me ntsis, Sonicated)

Fluorescent: λex 480 nm, λem 620 nm hauv dej

Refractive Index: 1.6700 (kwv yees)

Cia txias: Khaws ntawm chav tsev kub

Daim ntawv thov: Ethidium bromide(CAS: 1239-45-8)yog ib qho polyaromatic fluorescent me me molecule thiab nucleic acid intercalator dav siv los ua stain hauv DNA thiab RNA electrophoresis. Thaum intercalation rau hauv thaj av hydrophobic ntawm nucleic acid puag khub, fluorescence-quenching dej molecules cuam tshuam rau Ethidium bromide qauv raug tshem tawm thiab cov khi Ethidium bromide ces fluoresces heev zog piv rau dawb Ethidium bromide (~ 25 fold nce nyob rau hauv dsDNA). Intercalation distorts tus qauv ribonucleic acid thiab impedes ntuj DNA kev sib cuam tshuam, nthuav tawm lub zog mutagen cuam tshuam nrog Ethidium bromide. Qhov kev txiav txim siab ntawm DNA qauv kuj ua rau Ethidium bromide inhibitor ntawm DNA polymerase.

Siv:

1. Elliott, WH 1963. Biochem. J. 86: 562-567. PIB: 167489922

2. Lepecq, JB, et al. 1964. CR Hebd. Seances Acad. Sci. 259: 3: XIV. PIB: 14196160

3. LePecq, JB and Paoletti, C. 1967. J. Mol. Biol. 27: 87-106}. PIB: 6033613

4. Douthart, RJ, et al. 1973. Biochemistry. 12: 214-220}. PIB: 46302220

5. El Hamalawi, AR, et al. Xyoo 1975. Anal. Biochem. 67: 3 :ua. PIB: 11637633

6. Karsten, U. thiab Wollenberger, A. 1977. Anal. Biochem. 77: 3 :ua. PIB: 842829

7. Laine, B., et al. 1984. Biochem. Biophys. Res. Pawg. 119: 3: XIV. PIB: 6370250

8. Moore, SP thiab Sutherland, BM 1985. Anal. Biochem. 144: 15-19} ib. PIB: 28589899

9. Scaria, PV and Shafer, RH 1991. J. Biol. Chem. 266: 3: XIV. PIB: 20050888

Ntim & Xa

Pob: 5g / 10g / 25g / 50g / bulk pob

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